Preparation of Blood Agar
1-Suspend 28 g of nutrient agar powder in 1 litre of distilled water.
2-Heat this mixture while stirring to fully dissolve all components.
3-Autoclave the dissolved mixture at 121 degrees Celsius for 15 minutes.
4-Once the nutrient agar has been autoclaved, allow it to cool but not solidify.
5-When the agar has cooled to 45-50 °C, Add 5% (vol/vol) sterile defibrinated blood that has been warmed to room temperature and mix gently but well.
6-Avoid Air bubbles.
7-Dispense into sterile plates while liquid.