How to prepare blood agar medium in laboratory?

Preparation of Blood Agar

1-Suspend 28 g of nutrient agar powder in 1 litre of distilled water.

2-Heat this mixture while stirring to fully dissolve all components.

3-Autoclave the dissolved mixture at 121 degrees Celsius for 15 minutes.

4-Once the nutrient agar has been autoclaved, allow it to cool but not solidify.

5-When the agar has cooled to 45-50 °C, Add 5% (vol/vol) sterile defibrinated blood that has been warmed to room temperature and mix gently but well.

6-Avoid Air bubbles.

7-Dispense into sterile plates while liquid.