Which technique allows enumeration of living bacteria in a liquid culture?
a. Assay of cell mass by nephelometry
b. Estimation of DNA concentration
c. Microscopic count
d. Miles-Misra plate count
e. Weighing of total cell mass.
aanya.bansal Answered question August 14, 2024
The correct answer is (d) Miles-Misra plate count.
The Miles-Misra plate count, also known as the viable count or colony-forming unit (CFU) assay, is a technique used to enumerate living bacteria in a liquid culture. It involves:
1. Serially diluting the culture
2. Plating the dilutions on a solid medium (agar)
3. Incubating the plates to allow colonies to form
4. Counting the number of colonies, which represents the number of viable bacteria in the original culture
aanya.bansal Answered question August 14, 2024